Optimization of Liquid Ammonia Treatment for Enzymatic Hydrolysis of Miscanthus sinensis Anderss

Miscanthus biomass yield high, high photosynthetic efficiency, fast growth, easy breeding, widely distributed, is suitable as raw materials for the production of fuel ethanol. In this paper, the method of the pretreatment of liquid ammonia for Miscanthus, using the pretreatment of liquid ammonia to overcome biomass recalcitrance, adding cellulase enzyme, and the monosaccharide content is determined by using high performance liquid chromatography (HPLC). Effect of pretreatment process on the enzyme solution effect of awn, conducts the research to the water processing rate, temperature, residence time, amount of ammonia, the amount of H2O2 parameters. Data shows that, in the moisture content of 80%, temperature 130 ̊C, loading amount of ammonia 2:1, residence time 10 min, the glucan and xylan enzyme conversion rate are 73.23% and 73.28% respectively. After a mass balance, dry base mans per 100 g can get glucose 28.96 g and xylan 17.25 g.